human cytokine chemokine antibody array  (R&D Systems)

Journal: PLoS ONE

Article Title: Fibroblast Activation Protein (FAP) Is Essential for the Migration of Bone Marrow Mesenchymal Stem Cells through RhoA Activation

doi: 10.1371/journal.pone.0088772

Figure Lengend Snippet: (A) The protein levels of chemokines in FAP-depleted BM-MSCs were compared to the control with RayBio human chemokine antibody array 1, according to the manufacturer's manual. The corresponding chemokines spotted on the membrane was shown below the blot. The highlighted box in the blot indicated the position of GRO and GRO-α. Data are representative of two independent experiments. (B) As in (A) but with RayBio human inflammation antibody array 3. The highlighted box in the blot indicated the position of cytokines, IL-1β, IL-6, TGF-β and TNF-α, which are known to involve in cellular movement. Representative results of two independent experiments.

Article Snippet: Culture supernatants were collected and assayed using the RayBio human chemokine antibody array 1 and the RayBio human inflammatory marker antibody array 3 (RayBiotech, Norcross, GA, USA).

Techniques: Ab Array

Journal: BioMed Research International

Article Title: Anti-Inflammatory and Protective Effects of Juncus effusus L. Water Extract on Oral Keratinocytes

doi: 10.1155/2022/9770899

Figure Lengend Snippet: Effects of the Juncus effusus water extract on chemokine production in Porphyromonas gingivalis lipopolysaccharide- (LPS-) stimulated oral keratinocytes. RT-7 cells were pretreated with the J. effusus water extract (20-fold dilution) for 3 min. After the removal of the medium, RT-7 cells were cultured with P. gingivalis LPS (1 μ g/mL) for 24 h. The detection of chemokines in cell culture supernatants was performed using the Human Chemokine Antibody Array 1. (a) The results shown are representative images of two independent experiments with similar results. (b) A densitometric analysis of various chemokine productions. Bars indicate the relative densitometric intensities after the values were normalized with both positive and negative controls and background. In particular, positive controls were used to normalize the values from different membranes being compared. Values represent the means ± standard deviations of two independent experiments. ∗ P < 0.05 and ∗∗ P < 0.01 show significant differences between the indicated groups.

Article Snippet: Chemokine detection in cell culture supernatants obtained from RT-7 cells treated with P. gingivalis LPS and/or the J. effusus L. water extract was performed using the Human Chemokine Antibody Array 1 (Ray Biotech, Inc., Norcross, GA, USA), according to the manufacturer's instructions.

Techniques: Cell Culture, Ab Array